Supplementary Table 9. Methods used to estimate the male-specific ages of each of the MSY sequences and genes, shown in Figure 8.
Sequence or gene Genes and/or pseudogenes Ks Sources of age estimation Male-specific age (millions of years)
X-transposed TGIF2LY, PCDH11Y 0.011 Schwartz A, et al. Hum. Mol. Genet. 7, 1 (1998); Bohossian HB, Skaletsky H & Page DC. Nature 406, 622 (2000) 4
Chr 1 transposition PPP1R12BP 0.034 7
Chr 15 transposition CSPG4LY, GOLGA2LY 0.042 9
Chr 21 transposition TPTEP 0.047 10
Chr 13 transposition SLC25A15P, ADPRTL1P 0.070 14
Chr 3 transposition DAZ, KIAA0084P 0.147 Comparative mapping; Seboun E, et al. Genomics 41, 227 (1997) 30
Group 4$ AMELY, OA1P*, PRKY, ADLICANP*, ARSFP, ARSEP, ARSDP, GYG2P, KALP, VCY, NLGN4Y*, STSP 0.081 40 million years ago from Lahn BT & Page DC. Science 278, 675 (1997) 40
Chr 19 transposition ZNF381P 0.37 75
Group 3 ZFY, TBL1Y*, APXLP*, USP9Y, DBY, CASKP, UTY, TMSB4Y, OFD1P2*, CYorf15A*, CYorf15B*, EIF1AY, BCoRP* 0.28 Lahn BT & Page DC. Science 278, 675 (1997) 100
Group 2 SMCY 0.47 Lahn BT & Page DC. Science 278, 675 (1997) 150
TSPY TSPY 0.71 Vogel T, et al. Cytogenet Cell Genet 81, 265 (1998) 150
CDY CDY 0.75 150
Group 1 SRY, RPS4Y, RBMY 0.98 Lahn BT & Page DC. Science 278, 675 (1997) 280
Based on a Ks molecular clock calibrated by the chromosome 3 transposition. See the row for the Chr 3 transposition.
Based on the weighted average of Ks between DAZ and DAZL and between KIAA0084P and KIAA0084.
$ Approximately 3 Mb of MSY genomic sequence was assigned to Group 4 based on sequence divergence from homologous X-chromosomal sequence.
* Genes newly assigned to their group based on Ks estimates.